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. 2012 Jan 15;23(2):347–359. doi: 10.1091/mbc.E11-06-0568

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© 2012 Fukunaga et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Effect of Tbf1 and Rap1 cotethering on MRX association with nearby DNA ends. LacO₈-TetO₄-HO (KSC2863) and LacO₈-TetO₄-HO rif1Δ rif2Δ (KSC2864) cells expressing Mre11-myc and Tel1-HA were transformed with placI-TBF1, ptetR-RAP1, or the control vector, together with the GAL-HO plasmid. Cells were analyzed for Mre11 binding or Tel1 binding by ChIP assay as in Figure 1B. The LacO₈-TetO₄-HO construct is shown in Figure 4A. The strains used here contained a sir2Δ mutation to suppress inefficient HO cleavage resulting from Rap1 tethering nearby (Hirano et al., 2009).