Figure 3.
NF-κB regulates phospholipase Cγ2 (PLCγ2) phosphorylation, and protein kinase C (PKC) activity in collagen-activated platelets. Washed platelets (1.2 × 109/ml) were preincubated with or without BAY11-7082 (10 and 50 μM), followed by the addition of collagen (1 μg/ml) to trigger (A) IκBα degradation for 10 min or (B) PLCγ2 and (C) PKC substrate (p47) phosphorylation for 5 min. Cells were collected, and subcellular extracts were analyzed by immunoblotting. Data are presented as the means ± S.E.M. (n = 4). **p <0.01 and ***p <0.001, compared to the control group (resting); #p <0.05 and ###p <0.001, compared to the collagen group.