Figure 3.

Theoretical yields in rhamnolipid production with P. putida under different conditions. (a) Rhamnolipid yields on alternative carbon substrates. The yields were calculated for zero growth (black bars), zero growth and 30 mmol ATP/(g_CDW h) maintenance metabolism (white bars), growth at a rate of 0.4 1/h with 30 mmol ATP/(g_CDW h) maintenance metabolism (gray bars), and a growth rate of 0.8 1/h with 50 mmol/(g_CDW h) maintenance metabolism (shaded bars). The substrate uptake rates were constrained to 120 mCmol/(g_CDW h). (b) Rhamnolipid production capacity depending on biomass formation and maintenance metabolism for alternative carbon substrates. The course of rhamnolipid production for uptake of glucose (■), glycerol (●), sucrose (▲) and octanoate (♦) are displayed. The black curves display rhamnolipid production in dependence on the rate of growth; grey dashed curves display rhamnolipid production in dependence of maintenance metabolism.