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. 2011 Oct 17;10:80. doi: 10.1186/1475-2859-10-80

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Copyright ©2011 Wittgens et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Thin layer chromatography of rhamnolipids. The sample of P. aeruginosa PAO1 (lane 2), grown in PPGAS-medium at 37°C, contains mono- and di-rhamnolipid as does the commercial rhamnolipid (JBR425, Jeneil Biosurfactant Co.) (lane 1). P. putida, expressing the rhlAB operon of P. aeruginosa from the plasmid pVLT33_rhlAB, cultivated in glucose containing LB-medium produced mono-rhamnolipid (lane 4). Empty vector control pVLT33 (lane 3). The band located above the di-rhamnolipids is IPTG (i.e., lanes 3 and 4).