Skip to main content
. 2012 Jan 13;7(1):e28916. doi: 10.1371/journal.pone.0028916

Figure 5. The CV1-derived nuclear protein that recognizes the DNA sequence between nt. 626/640 is not AP-1.

Figure 5

A. Sense sequence of FFL14 oligo DNA probe encompassing nt. 613/652, its mutant (FFL14G), and AP-1 oligo DNA probe [70]. The sequence that has weak homology with AP-1 site in FFL14 oligo and consensus Jun/Fos binding sequence in AP-1 are indicated in boldface. FFL14G that has mutations in the sequence similar to AP-1 site was used as control for competition assay. B. Gel shift assay using radiolabeled FFL14 with CV1 nuclear extract (CV1-NE). Arrow indicates the specific binding. C. Gel shift assay using radiolabeled AP-1 probe [70] with CV1-NE. Arrowhead indicates the specific binding containing cJun. The binding signal was abolished by the addition of anti-cJun antibody (lanes 5 and 6). D. Gel shift assay using radiolabeled FFL14 probe with CV1-NE in the presence of anti-cJun antibody. The binding signal (arrow) was not affected by the addition of anti-cJun antibody (lanes 5–7). NS, non-specific cold competitor.