Table 6. Primers used for reverse transcription quantitative PCR (RT-qPCR).
| Gene | Primer sequences | ||
| Name | Locus tag | Forward | Reverse |
| ald | PFREUD_00370 | TGTTCACCTACCTGCACCTG | GAGCTCAACCGTCTCGTAGG |
| argG | PFREUD_01460 | GACCATCAACGGCAAGAAGT | TCGTAGGCGATGAACAACAG |
| argJ | PFREUD_13980 | GCATGTCCACGAACGACTC | AACTGGTGTCCCAGGTTGAG |
| aspA2 | PFREUD_16330 | TCCACGCGTACAGAAGAAGA | CCGGACATCTGGAAGTTCTC |
| bkdA2 | PFREUD_02200 | TCGCATTTCGTACACACTCC | TTCGGCTCGAAGAAGATCAC |
| cspB | PFREUD_18210 | GCGATGATGGAGGATTTGAT | CGATCTTTGAGGCCTGACC |
| cstA | PFREUD_16500 | CCTACACACCGGAGGAAGAG | GGACCGTCCATTTGTTATGC |
| dps | PFREUD_02870 | GACCTGGTCGTGAAGTCGAT | GCTTGTTGATCAGCTCATGG |
| eno1 | PFREUD_17320 | TACGAGTTCGAGGGCAAGTC | AAACCAGCGGGTAGTCATTG |
| fba2 | PFREUD_23890 | GATCAGAAGGACCGGATGG | CATTGTCGTAGGCATCATCG |
| feoA | PFREUD_19660 | AGACCGTGGGTTCCAAGAT | GACTCGTCAGCCTCTTCGTC |
| feoB | PFREUD_19650 | GCGGTGATGTTCCTGTTCTT | TAGCCGAAGAAGGTGTCGAT |
| ftsX | PFREUD_09600 | AGAACTACAAGGGCGTGGTG | CCGTTGAGGACCTTGAAGAA |
| fumC | PFREUD_16300 | CCCAACCTCGAGAAGATCAA | ATCTTCGAGGCCTTGTCGTA |
| glgC | PFREUD_16180 | CGTGCTCACGCAGTACAAGT | GGTACGGGCGTCACATAGTT |
| groeS1 | PFREUD_06470 | CAATGCGGTGCTTCTTCTC | GGCAAGGACTCCGATGTG |
| gtfF | PFREUD_19370 | TGGTGACGCCGAAGGACTC | GCAGCACGAGCAGGAACAC |
| gyrB1 | PFREUD_12820 | TTGCAGGGCAGCGACCACTT | GACCAGCGCACGGCAACAT |
| ldh2 | PFREUD_12840 | GATGCCTCCACCAATGAGAT | CATAGTTCGTGGAGCCCTTG |
| livG | PFREUD_10850 | TCTGCGAGCGTATCTACGTG | GGTATGCCTCGATCACCTGT |
| mraZ | PFREUD_15590 | ATCAATCGGGTTGAGGTGTG | GTTCATCTGGGCGAAGACAT |
| PFREUD_18300 | PFREUD_18300 | CACGGAGGAGGAGAAGATCA | GTACTTCCACAGCTGCACGA |
| PFREUD_20370 | PFREUD_20370 | ACCTGACCTTCGACATGACC | GGGGTACTGAGTGGAGGTGA |
| pgi | PFREUD_04290 | GAGGCACTCAAGCCCTACAA | AGGTCGTGGGTCTTCTCGTA |
| pgm1 | PFREUD_10610 | CCGAGGTGGAGTTCTTCAGT | GACCAGCTTGGACGACAAC |
| ppa | PFREUD_23500 | TGAAGTCGGTTGATGGTGAG | GTTCCAGCTCCTGCTTCATC |
| ppdk | PFREUD_03230 | ATGCCTCGATGAAGTCCTTG | GTGGTGACCGTAAAGCCATC |
| pf279-F1 | PFREUD_04340 | CGACTCCTACCAGCAGAAGC | CATTGTTTGACAAGGCCTGA |
| pspC | PFREUD_06710 | GATCTGCTGGCTGTTGATCC | TCGTAGGGGTTGAAGTCCTG |
| ptsI | PFREUD_19470 | CGAGATCAAGAACGACACCA | GGTTGAAATAGGCGTTCGAC |
| sdaA | PFREUD_18570 | CGCGGAACTGCTCAATATCT | CACCTCGTCCTTCGAGTAGC |
| sdhC2 | PFREUD_14320 | CTGATGGCTCTCACCGGTAT | TCGTTCATGAGCAGCTTCAG |
| ND | PFREUD_18870 | CATGGCTGAAAACAACAATTTG | TCTGCTTACCCTGGGAATG |
| tuf | PFREUD_05650 | CGAACGAGTTCCACTGCGGGT | GCAACATCGGCACCATCGGAC |
| tyrB | PFREUD_09460 | CGACATGGACTTCATCAACG | ATCGGTGCCATAGACATGGT |