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. 1983 Mar 25;11(6):1645–1655. doi: 10.1093/nar/11.6.1645

pEMBL: a new family of single stranded plasmids.

L Dente, G Cesareni, R Cortese
PMCID: PMC325826  PMID: 6300771

Abstract

We have constructed a series of plasmids, the pEMBL family, characterized by the presence of 1) the bla gene as selectable marker, 2) a short segment coding for the alpha-peptide of beta-galactosidase and containing a multiple cloning sites polylinker, 3) the intragenic region of phage F1. pEMBL plasmids have the property of being encapsidated as single stranded DNA, upon superinfection with phage F1. These vectors have been used successfully for DNA sequencing with the dideoxy-method, and can be used for any other purpose for which M13 derivatives are used. However, the pEMBL plasmids have the advantage of being smaller than M13 vectors, and the purification of the DNA is simpler. In addition, and most importantly, long inserts have a higher stability in pEMBL plasmids than M13 vectors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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