Table 1.
Condition | Base medium | Supplementation | Viability loss |
---|---|---|---|
LBTMP | Luria-Bertani broth | - | + (90% loss in 3 hours) |
M9TMP | Minimal M9 | None | - |
M9TMPAdAA | Minimal M9 | Met, Gly, Ad | + (>70% loss in 6 hours) |
M9TMPAA | Minimal M9 | Met, Gly | - |
M9TMPAd | Minimal M9 | Ad | - |
M9TMPThyAdAA | Minimal M9 | Met, Gly, Ad, Thy | - |
A list of treatment regimens used. Viability loss indicates the relative magnitude of cell lethality (+: maximum, -: no loss). TMP: Trimethoprim (50 or 25 μg/ml), Met: methionine, Gly: glycine, Ad: adenine (each at 50 μg/ml final concentration)