Table 2.
List of mutants yielding high biomass in presence of TMP at sub-lethal concentrations in LB medium
| Resistant knock-out candidates | ||
|---|---|---|
| Gene | Gene ID | Description |
| moaE | b0785 | Molybdopterin (MPT) synthase, large subunit; chlorate resistance; dimer of dimers with MoaD |
| ybhN | b0788 | Function unknown |
| yliE | b0833 | Function unknown |
| ymfQ | b1153 | Function unknown |
| yobG | b1826 | Function unknown |
| yecN | b1869 | Function unknown |
| crr | b2417 | Phosphocarrier protein for glucose of the PTS; Enzyme IIA(Glc); formerly EIII(glc) |
| recJ | b2892 | Single-stranded DNA-specific exonuclease, 5'-3' |
| ygjT | b3088 | Function unknown; induced by alkali; putative membrane transport or efflux protein |
| ygjU | b3089 | Na+/serine (threonine) symporter |
| nanA | b3225 | N-Acetylneuraminate lyase (aldolase) |
| yhiP | b3496 | Function unknown |
| uhpT | b3666 | Fosfomycin sensitivity; sugar P transport system; transport protein for hexose P's |
| yiiQ | b3920 | Function unknown |
| uvrA | b4058 | Excision nuclease subunit A; repair of UV damage to DNA; LexA regulon; binds Zn(II) |
| fimE | b4313 | Site-specific recombinase for fimA promoter segment inversion; bias for ON to OFF phase switching |
| deoC | b4381 | Deoxyribose-phosphate aldolase; deoxyriboaldolase; binds selenium |
| deoA | b4382 | Thymidine phosphorylase |
A total of 18 mutants, based on their relatively high biomass yield in presence of TMP, were identified as sensitive candidates. Genes whose functions are related to DNA maintenance and nucleotide salvage pathway are highlighted in bold. Genes are sorted by their Gene IDs.