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Elastin protein levels in lysates of embryonic day 19 cultured fetal rat lung fibroblasts for 24h in either DMEM + 10% fetal bovine serum, or in DMEM with reduced (5, 1, or 0 %) fetal bovine serum supplementation are shown. Serum restriction increased elastin protein levels dose-dependently, shown by Western blotting. (*p<0.05 vs. 10% FBS; n=3).
