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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Alcohol. 2011 Sep 17;46(1):3–16. doi: 10.1016/j.alcohol.2011.08.003

Fig. 2.

Fig. 2

CEE does not alter the integrity of the cytoskeleton of astrocytes contained in young or old CGCs cultures. Co-cultures of CGCs and astrocytes were treated with 100 mM ethanol as described in the materials and methods section. Cells were then processed for identification of the cytoskeleton with GFAP / Cy3 at four different times post-CEE and micrographs were acquired with a confocal microscope. Note the lack of change in the morphology of the astrocytic cytoskeleton for (A) control, (B) Day 5 and at (C) 72+W for young DIV co-cultures of astrocytes and CGCs. Representative images for CEE started at 21+ DIV cultures of CGCs and astrocytes are shown for (D) control, (E) Day 5 and (F) 72+W also depict no change in astrocytic morphology attributed to CEE. Summarized data for young and old DIV co-cultures are shown in graphs G and H, respectively as percent change from control values; scale bar at 20 µm.