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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Alcohol. 2011 Sep 17;46(1):3–16. doi: 10.1016/j.alcohol.2011.08.003

Fig. 7.

Fig. 7

CEE did not increase cell death of CGCs expressed in young or old DIV cultures. The percentage of dead cells as indicated by an uptake of the trypan blue dye compared to non-blue cells was measured as presented in the Materials and methods section. Paired t test indicated no significant differences for young DIV CGCs at any time point post CEE compared to culture matched controls: t = 3.8, P = 0.7; t = 1.1, P = 0.3; t =1.9, P = 0.1 and t = 1.6, P = 0.2 for Day 5 and for the 24+W, 48+W and 72+W time points, respectively. Similar results were observed for experiments conducted on old DIV CGCs: t = 0.6, P = 0.5 for cells at Day 5; t = 0.3, P = 0.7 at the 24+W time point; t =1.3, P = 0.3 at the 48+W time point, and t = 1.0, P = 0.3 for the 72+W time point. Summarized data for old DIV CGCs (A) and young DIV CGCs (B) are depicted as percent non-viable cells (cells that took up the dye and were therefore blue) compared to values from control cells.