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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Physiol. 2012 Jun;227(6):2632–2644. doi: 10.1002/jcp.23005

Fig. 4. H2O2 inhibits gene and protein expression of RARα and RXRα.

Fig. 4

Cardiomyocytes were exposed to 100 μM of H2O2 for different time periods, and nuclear protein (A & B) and gene (C) expression of RARα and RXRα was determined. Each value represents the mean ± SEM (n = 3). *, p<0.05, versus control. D & E. Cardiomyocytes were transfected with pRXR-Luc and pRAR-Luc, for 6 h, and exposed to 1 μM of 9-cis RA (9-cis) and ATRA (RA), in the absence or presence of 100 μM of H2O2, for 12 h. RARE and RXRE-dependent luciferase activity was measured, as described in Fig. 1. Each value represents the mean ± SEM (n = 3). *, p<0.05, versus control; #, p<0.05, versus HG.