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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Exp Hematol. 2011 Oct 21;40(2):131–142.e4. doi: 10.1016/j.exphem.2011.10.006

Fig. 1.

Fig. 1

Ex-vivo culture of p53−/− progenitor cells with Tpo generates hyperploid megakaryocytic cells in part due to enhanced DNA synthesis. (A) Percent of CD41+ Mk cells on day 6 of culture of p53+/+ and p53−/− pronenitor cells with 50 ng/ml rm Tpo. Error bars: SEM, N=7, * represents P < 0.02, and (B) averaged ploidy data for p53+/+ and p53−/− CD41+ Mk cells on day 6 of Tpo culture. Error bars: SEM, N=7. (C) Representative DNA synthesis data from p53+/+ and p53−/− cultured Mk cells and (D) average of the DNA synthesis data for day 4. Error bars: SEM, N=3–4, * indicates P < 0.03. Polyploidization among the cycling CD41+ Mk cells (BrdU+) on day 4 of Tpo culture. (E) Representative ploidy histograms from BrdU+ p53+/+ and p53−/− cultured Mk cells and (F) average of the % ≥32N polyploid cells among BrdU+ Mk cells for day 4. Error bars: SEM, N=3–4.