Skip to main content
. Author manuscript; available in PMC: 2013 Jan 10.
Published in final edited form as: Neuroscience. 2011 Nov 17;201:34–45. doi: 10.1016/j.neuroscience.2011.11.021

Figure 4. RNA interference knocks down 14-3-3-θ in NIH/3T3 cells.

Figure 4

Three siRNAs were tested for their potency in reducing in vitro transcription and expression of 14-3-3-θ. A. PCR-amplified 14-3-3-θ transcripts and cyclophilin (cyclo) transcripts in mouse brain and in NIH/3T3 cells are shown. Transcripts in Lane 0 are from a mouse brain lysate. Transcripts from NIH/3T3 cells treated with three different siRNAs are shown in the other lanes. Treatment with s479 produced maximal reduction of 14-3-3-θ. B. Western blots were immunoprobed with antibodies to 14-3-3-θ, with β-actin serving as a loading control. Maximal reduction in the expression of 14-3-3-θ is also shown with s479. C. Transfection efficiency was determined using a control fluorescent oligonucleotide. D. Expression of 14-3-3-θ was tested in untransfected NIH/3T3 cells. All cells were immunolabeled by the 14-3-3-θ antibody. E. Transfection of NIH/3T3 cells with s479 reduced expression of 14-3-3-θ.