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. 2011 Jun 8;21(2):217–227. doi: 10.1089/scd.2011.0143

FIG. 1.

FIG. 1.

PUM2 production in ASCs, UCB-, and BM-derived MSCs. (A) RT-PCR analysis of pum2 expression in MSCs from different sources and ASCs. Numbers indicate different donors. Expression of the GAPDH and RNApolII genes was used as a control. (B) Western blot analysis with anti-hPUM2 (114 kDa) antibody on protein extracts from cells derived from 3 sources: UCB-MSC, BM-MSC, and ASC. The β-actin (43 kDa) protein was used as a loading control. (C) Indirect immunocytochemical studies showing the distribution of PUM2 in ASCs. Scale bar=40 μm. (D) Flow cytometry quantification of PUM2+ cells: the control was cells incubated with FITC-conjugated isotype antibodies used to set the gate. A dot plot of the population of ASC from 2 donors (AD1 and AD2) is displayed. Fluorescence intensity (FL2-H channel) is shown on the y-axis on a log (100–104) scale and fluorescence intensity (FL1-H channel) is shown on the x-axis, on a log (100–104) scale. ASCS, adipose-derived stem cells; BM, bone marrow; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MSCs, mesenchymal stem cells; PUM2, Pumilio-2; UCB, umbilical cord blood.