FIG. 3.

Identification of Pum2-associated mRNAs. (A) IP-western blot of PUM2, probed with anti-PUM2-specific antibody. IP-PUM2 and IP-control (negative control); (B) mRNA associated with PUM2. Rows represent unique transcripts and the color code indicates the degree of enrichment. Two experiments with Pum2 protein (AD54 and AD55) and 2 negative controls are shown. (C, D) RT-qPCR of anti-PUM2 immunoprecipitated mRNAs. Transcript levels in IP-PUM2 versus either negative IP (IP-control), normalized to concentration, are shown. Analysis of enrichment of target (fzd7and klf6) of IP-PUM2 ASC from 5 donors (AD54, AD55, AD59, AD60, and AD61). IP, immunoprecipitation. Color images available online at www.liebertonline.com/scd