FIG. 6.

GFP expression was retained in insulated clones after directed differentiation toward mesodermal lineages. Markers that characterized mesodermal lineages such as SMA (A–C) colabeled with GFP after differentiation. Cells expressing cardiovascular markers PDGFRα (D–F) and Brachyury (G–I) also retained robust GFP expression. PECAM1 (CD31), a common marker for endothelial cells, was detected to be coexpressed with GFP (J–L, inset in L shows the phase image of J–L). In addition, single cells dissociated from beating clusters were analyzed by flow cytometry and showed coexpression with GFP (M). Black curve represents a control cell line that does not have GFP expression; green curve represents the iCAGG-derived beating cells. Quantification of coexpressed cells of GFP and mesodermal lineage markers is shown in (N). Scale bar, 100 μm. Color images available online at www.liebertonline.com/scd