Table 2.
Infection Status, Treatment Regime, SIV Levels and Clinical Status at Time of Death and Necropsy of Monkeys
| Monkey No | SIV infection† | Drug treatment‡ | SIV in tissues§ | SIV in fluids# | Clinical status¶ | ||
|---|---|---|---|---|---|---|---|
| Ln/Spl | Brain | Plasma | CSF | ||||
| 44 | − | Not treated | ND | ND | ND | ND | No disease |
| 50 | − | Not treated | ND | ND | ND | ND | No disease |
| 69 | − | Not treated | ND | ND | ND | ND | No disease |
| 87 | − | Not treated | ND | ND | ND | ND | No disease |
| 75 | 2.5 | Not treated | + | − | − | n.a. | Asymptomatic |
| 80 | 6.5 | Not treated | + | − | − | n.a. | Asymptomatic |
| 85 | 4.5 | Not treated | ++ | − | − | n.a. | Asymptomatic |
| 92 | n.a. | Not treated | + | − | − | n.a. | Asymptomatic |
| 93 | 4.5 | Not treated | + | − | − | n.a. | Asymptomatic |
| 46 | 19 | Not treated | + | + | +++ | n.a. | AIDS |
| 71 | 6.5 | Not treated | ++ | ++ | ++ | n.a. | AIDS |
| 74 | 6 | Not treated | +++ | +++ | ++ | n.a. | AIDS |
| 78 | n.a. | Not treated | +++ | ++ | + | n.a. | AIDS |
| 79 | 2.5 | Not treated | + | + | n.a. | n.a. | AIDS |
| 82 | 3 | Not treated | +++ | +++ | +++ | +++ | AIDS |
| 86 | 4.5 | Not treated | +++ | +++ | n.a. | n.a. | AIDS |
| 70 | 6.5 | ddI/6-Cl-ddG | + | − | + | − | AIDS |
| 72 | 8 | ddI/6-Cl-ddG | −/+ | − | + | − | AIDS |
| 73 | 9 | ddI/6-Cl-ddG | +/++ | −/+ | + | + | AIDS |
| 83 | 4 | ddI/6-Cl-ddG | +/++ | − | ++ | − | AIDS |
| 89 | 22 | 6-Cl-ddG | ++ | − | +++ | ++ | AIDS |
| 91 | n.a. | ddI/6-Cl-ddG | + | − | +++ | + | AIDS |
Inoculation with SIV strain δB670. Time of infection and sacrifice is presented in months.
Treatment with ddI before with 6-Cl-ddG. Monkey 89 was treated only with 6-Cl-ddG.
Detection of SIV by immunohistochemistry and in situ hybridization in tissues. Sections from several lymphoid organs (axillar, inguinal and mesenterial lymph nodes, and spleen) and brain regions (frontal and parietal cortex, hippocampus, and basal ganglia) were analyzed for SIV gp120 and SIV RNA, summarized and scored as follows: − (no positive cells) to +++ (>100 cells/mm2).
Detection of SIV by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction in plasma and CSF at sacrifice. Plasma of asymptomatic monkeys (Nos. 75, 80, 85, 92 and 93) and AIDS monkeys (46, 71, 74, 78 and 82) were analyzed for SIV p26. CSF and plasma of untreated AIDS monkey 82 and antiretrovirally treated AIDS monkeys (70, 72, 73, 83, 89 and 91) were analyzed for SIV RNA. Scoring for SIV p26 was as: −(<1 ng/mL) to +++ (>30 ng/mL). SIV RNA was normalized to SIV particle number and scored as: −(<50 particles/mL) to +++ (>105 particles/mL).
Criteria defining AIDS included 1 or more of the following: anemia, loss of body weight over 10%, intractable diarrhoea/dehydration requiring fluid replacement, oral lesions/thrush and other opportunistic infections.
Abbreviations: AIDS, acquired immunodeficiency syndrome; 6-Cl-ddG, 6-chloro-2′.3′-dideoxyguanosine; CSF, cerebrospinal fluid; ddI, 2′,3′-dideoxyinosine; Ln, lymph nodes; n.a., not applied; ND, not done; No, number; SIV, simian immunodeficiency virus; Spl, spleen.