Figure 1. Rolipram treatment slows epithelial cell shedding.
(A) 24 hour migration of BrdU labeled cells was quantified in mice treated with rolipram. *, P≥0.05 compared to untreated control mice. Values are mean±SEM of 3 separate experiments. (B) Micrographs of ileum of adult mice corresponding to panel A. Bars represent BrdU positive cells. (C) Acriflavin stained intestinal epithelium was wounded using a 2-photon laser (wounded area denoted with white arrow). Increased time to shedding was observed in rolipram-treated animals. Images were taken at 400×. Images are representative of 3–4 mice.