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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Inflamm Bowel Dis. 2011 Oct 12;18(6):1081–1091. doi: 10.1002/ibd.21898

Figure 5. cAMP decreases epithelial cell migration through activation of protein kinase A.

Figure 5

(A and B) Quantification of cell migration rate in untreated cells (open circles) and cells treated with the protein kinase A (PKA) activator, 6-Bnz-cAMP (solid boxes). Cell migration is significantly decreased in cells at the (A) leading edge of the wounded epithelium as well as cells 10 rows back in the (B) monolayer. Mean±SEM, n = 3; *, P≤0.05 from untreated cells. (C) Pharmacological inhibition of PKA with H89 [1µM] reversed the negative effect of 10µM forskolin (FSK) on epithelial migration, with re-establishment of migration to levels consistent with untreated cells. Values are mean±SEM, n=4; *, P≤0.05.