Table 1.

miRNA expression in B6.Sle123 B and T lymphocytes

miRNA	2 mo. (mild disease)		6 mo. (moderate disease)		12 mo. (severe disease)
	RQ1 ± SEM	p-value	RQ1 ± SEM	p-value	RQ1 ± SEM	p-value
A. Differential miRNA expression in B6.Sle123 splenic B Cells
miR-21	1.7 ± 0.15	0.001	2.2 ± 0.42	0.036	5.8 ± 0.64	0.016
miR-34a	0.9 ± 0.38	0.670	1.2 ± 1.33	0.821	13.2 ± 0.65	0.000
miR-146a	1.0 ± 0.51	0.996	0.6 ± 0.25	0.044	5.3 ± 0.75	0.032
miR-221	1.2 ± 0.29	0.504	1.2 ± 0.28	0.398	13.3 ± 1.05	0.023
miR-222	1.4 ± 0.22	0.040	2.1 ± 0.26	0.015	5.8 ± 0.96	0.037
miR-223	0.9 ± 0.53	0.720	2.3 ± 1.61	0.507	11.4 ± 0.68	0.006
miR-142-5p	1.1 ± 0.21	0.374	0.4 ± 0.34	0.017	2.7 ± 0.45	0.032
miR-155	1.3 ± 0.12	0.026	1.2 ± 0.39	0.504	2.8 ± 0.53	0.050
miR-150	0.7 ± 0.45	0.314	0.5 ± 0.27	0.098	0.5 ± 0.18	0.006
miR-181a	0.5 ± 0.20	0.007	0.3 ± 0.78	0.069	0.4 ± 0.36	0.020
B. Differential miRNA expression in B6.Sle123 splenic naïve T cells
miR-21	2.5 ± 0.37	0.012	3.6 ± 1.00	0.110	3.0 ± 0.58	0.032
miR-34a	1.5 ± 0.91	0.528	4.2 ± 1.05	0.094	6.2 ± 0.50	0.006
miR-146a	1.0 ± 1.20	0.955	4.9 ± 1.56	0.216	4.1 ± 0.31	0.002
miR-221	0.3 ± 0.47	0.013	2.3 ± 1.40	0.433	4.0 ± 0.39	0.002
miR-222	0.6 ± 0.49	0.187	1.2 ± 0.50	0.672	0.5 ± 0.20	0.006
miR-223	1.2 ± 0.55	0.697	4.6 ± 0.58	0.019	11.1 ± 0.23	0.000
miR-142-5p	1.0 ± 0.33	0.906	1.3 ± 0.56	0.550	2.6 ± 0.46	0.027
miR-155	0.5 ± 0.12	0.000	2.1 ± 1.40	0.475	2.1 ± 0.17	0.000
miR-150	1.0 ± 0.84	0.943	0.7 ± 0.69	0.553	0.6 ± 0.22	0.008
miR-181a	1.4 ± 0.77	0.562	1.0 ± 0.97	0.980	0.2 ± 0.66	0.015
C. Differential miRNA expression in B6.Sle123 splenic memory T cells
miR-21	2.3 ± 0.26	0.00371	2.3 ± 0.32	0.00935	2.9 ± 0.31	0.00734
miR-34a	1.2 ± 0.46	0.61619	2.8 ± 0.98	0.17000	6.1 ± 1.0	0.03999
miR-146a	2.0 ± 0.27	0.00815	1.9 ± 0.23	0.00692	1.8 ± 0.31	0.03301
miR-221	0.9 ± 0.29	0.78955	2.2 ± 0.33	0.02462	3.5 ± 0.59	0.01522
miR-222	3.0 ± 0.66	0.04896	2.5 ± 0.39	0.02568	4.7 ± 0.91	0.03822
miR-223	1.1 ± 0.44	0.80584	1.7 ± 0.14	0.00425	5.3 ± 0.89	0.02602
miR-142-5p	1.2 ± 0.22	0.35640	1.3 ± 0.39	0.38912	0.9 ± 0.52	0.72454
miR-155	0.1 ± 0.86	0.02879	1.3 ± 0.30	0.91145	0.1 ± 0.31	0.00023
miR-150	1.3 ± 0.36	0.34351	1.1 ± 0.50	0.80519	1.0 ± 0.68	0.92495
miR-181a	0.8 ± 0.33	0.49138	0.9 ± 0.98	0.93433	0.2 ± 0.79	0.01509

A. CD19⁺ splenic B cells from individual B6.Sle123 and age- and gender-matched B6 mice at 2, 6 and 12 months of age were FACS purified. Twenty nanograms total B cell RNA were reverse-transcribed with TaqMan miRNA-specific reverse-transcription primers followed by quantification using Taqman miRNA-specific qRT-PCR assays. B6.Sle123 miRNA RQ values represent the average of triplicate measurements from at least three independent experiments in individual B6.Sle123 mice relative to the expression of the same miRNA in individual B6 B cells. Prior to obtaining RQ values, the C_t values for each miRNA in each sample, were normalized against sno429 expression (ΔC_t). The mean B6.Sle123 miRNA RQ was calculated by evaluating where ΔΔC_t = ΔC_{t Sle123 ave} − ΔC_{t B6 ave}.

B. As in A except total splenic CD44^Low CD62L^High naïve T cells were FACS purified from individual mice.

C. As in A except total splenic CD44^High CD62L^Low memory T cells were FACS purified from individual mice.