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TNF and GSK3 regulate chromatin accessibility at the IL6 promoter. (a) Schematic representation of the proximal IL6 promoter. (b) Human macrophages were pretreated for 24 hr with LPS or TNF with or without SB216763 (10 µM) and then challenged with 10 ng/ml LPS for 3 hr. Nuclei were digested with 50 U of BsrBI for 20 min at 37°C. Purified genomic DNA was analyzed by Southern blot. Data shown are representative of three independent experiments.
