Figure 2.

Presence of both inf-HIV and non-inf HIV impairs the ability of DC to prime naïve T-cell responses. IDC and MDC were pulsed with inf-HIV or AT-2 HIV (either HIV-1MN (CXCR4-tropic) or HIV-1BaL (CCR5-tropic)) overnight, washed twice, and cocultured with CFSE-labeled naïve bulk T cells at a ratio of 1:10 (10⁴ DC and 10⁵ T cells). Priming cultures were restimulated with 10 000 DC/well after 7 days of coculture and T-cell proliferation measured on day 8 by CFSE dilution by flow cytometry or by ³H-Thymidine incorporation. (A) HIV-1MN effects on the ability of MDC and IDC to induce T-cell proliferation (figure shows one experiment with suppression of T-cell proliferation). Background proliferation with T cells alone ranged from 3 to 8% depending on the donor. (B) HIV-1BAL effects on the ability of MDC and IDC to induce T-cell proliferation (graph shows one representative experiment). (A and B) Bars show the mean of three wells per group±SEM. (C and D) Normalized values for assays performed with CXCR4-tropic HIV-1MN (n=8) and CCR5-tropic HIV-1BaL (n=20) respectively. ^***p<0.001, one-way ANOVA non-parametric Tukey's test.