Figure 3.

Discrimination of innate immune and IFN-γ-stimulated gene expression signatures in monocyte-derived macrophages. (A) Upregulation of gene expression in monocyte-derived macrophages (MDMs) stimulated for 4–24 h with Mtb (as an innate immune stimulus) or with 10 ng/mL IFN-γ. Three separate experiments from different donors for each stimulation group were compared with eight separate experiments from different donors for unstimulated MDMs. (B) All significant gene expression differences were aligned in an expression matrix representing mean fold change compared with unstimulated MDMs. (C) The differences in transcriptional profiles from unstimulated MDMs and Mtb or IFN-γ-stimulated MDMs were also assessed by PCA (data points represent mean±SEM of PC scores).