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. 2010 Dec 23;519(6):1007–1023. doi: 10.1002/cne.22570

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Copyright © 2011 Wiley-Liss, Inc., A Wiley Company

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NPY and VGAT in the superficial dorsal horn. a–c: Confocal images of a single optical section showing part of lamina I in a transverse section that had been reacted to reveal NPY (green) and VGAT (magenta). Several NPY-immunoreactive axonal boutons are visible, and some of these are indicated with arrows. As seen in the merged image (c), all of these boutons are also VGAT-immunoreactive, but they are surrounded by many other VGAT-positive boutons that lack NPY. The very fine green profiles (three indicated with arrowheads) were found to be intervaricose portions of NPY-immunoreactive axons when followed through the series of optical sections, and these did not contain VGAT. d: A projection of 24 optical sections at 0.3 μm z-spacing from the same field shows the distinction between boutons (arrows) and intervaricose portions (arrowheads) of NPY-immunoreactive axons. Scale bar = 5 μm.