Figure 3.

Effect of Taxol on ILK, collagen, phosphorylated Smad2/3 (p-Smad) and α-SMA expression in the rat remnant kidney. (A) Real-time PCR analyses revealed that Taxol inhibited the expression of ILK, COL(I)A1, COL(IV)A2 and α-SMA in the rat remnant kidney (p < 0.05, n = 12). No differences in Smad2 and Smad3 mRNA expression were observed in various experimental groups. (B) Immunofluorescence microscopy showed that expression of COL(I)A1 and COL(IV)A2 was significantly increased in the remnant kidney (p < 0.05, n = 12) and notably reduced with Taxol treatment (p < 0.05, n = 12). (C) Immunohistochemistry showed that expression of p-Smad2/3 and α-SMA was significantly increased in the remnant kidney (p < 0.05, n = 12) and notably reduced with Taxol treatment (p < 0.05, n = 12). Data of each bar represents mean ± SEM for 12 animals. ^Δp < 0.05 versus Sham group (n = 12); ^#p < 0.05 versus Rem group. Magnification, × 400