Fig. 5.

Injection of hAAT inhibits in vivo proinflammatory cytokine production after BMT. B6D2F1 mice were exposed to 1,000 cGy of total-body irradiation and transplanted with 5 × 10⁶ T-cell–depleted BM cells and 2 × 10⁶ T cells from either allogeneic (B6) or syngeneic (B6D2F1) donors. Each F₁ recipient of the allogeneic cells was injected i.p. with 4 mg hAAT or human albumin on days −2, +1, and +4. Sera from the recipient animals (n = 4–5/group) were obtained on day 7 after BMT and analyzed for TNF-α, IL-1β, and IL-6. Albumin-treated allogeneic controls (solid bars) vs. hAAT allogeneic recipients (open and gray bars) for TNF-α. *P < 0.04, IL-1β; Allo, **P < 0.03; IL-6, *P < 0.04.