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. 2011 Dec 27;109(2):535–540. doi: 10.1073/pnas.1119351109

Fig. 3.

Fig. 3.

Aire accumulates at the TSS of Pol-II–rich genes. (A) ChIP-seq analysis was performed in control or Aire-transfected 293 cells. Binding of Aire (Upper) and total Pol-II (Lower) over the whole length of an Aire-induced (THAP10) and an Aire-neutral gene (ZBTB25), shown as examples. (B) As in A, close-up representation of the TSS region of an Aire-induced (INTS12) and an Aire-neutral gene (NME1). (C) Cumulative distribution of Aire and Pol-II in a 4-kb window around the TSS of 2,272 genes. (D) Distribution of total Pol-II and Aire in 25-bp bins in a window of 4 kb around the TSS of all genes, ranked by expression values. (E) As in D, representation of Aire-bound DNA in an independent experiment at two different times after Aire transfection; Right: chromatin precipitated with control IgG (normal mouse IgG1).