Skip to main content
. 2011 Dec 21;109(2):466–471. doi: 10.1073/pnas.1118857109

Fig. 3.

Fig. 3.

Differential responses of Lgr5+ vs. Bmi1+ lineages to acute radiation injury. (A and B) Tamoxifen-mediated lineage trace of Lgr5-eGFP+ progeny in Lgr5-eGFP-IRES-CreERT2; Rosa-YFP mouse duodenum demonstrates prolific lineage generation under homeostasis by 8 d (A). Lgr5-eGFP+ ISCs and their lineage are ablated at 7 d after 12 Gy whole-body irradiation (B). (C and D) Bmi1+ lineage in Bmi1-CreER; Rosa-YFP duodenum marked by 9-d tamoxifen treatment exhibits marked proliferative regenerative response with progeny observed in adjacent crypts and villi at 7 d after irradiation. (Scale bars; A–D, 100 μm.) Phalloidin is shown in red and DAPI in blue. (E–G) 3D confocal reconstruction of Bmi1+ lineage regenerative response in the jejunum at 7 d after 12 Gy irradiaton highlights confluent patches of Bmi1+-derived cells in multiple adjacent crypts/villi. Zoomed view of regenerated jejunum immunostained with lysozyme, illustrating Paneth cells within a budding crypt (F). Phalloidin is colored in red and DAPI in blue. (H–K) Monoclonal repopulation of 7 d postirradiated jejunum from Bmi1-CreER; Rosa-Confetti compound heterozygotes, as suggested by monochromatic patches of regenerated crypts/villi illustrated in RFP (H), GFP (I), CFP (J), and YFP (K). Insets represent serial cross-sections through lineage traces indicating involvement of multiple contiguous crypts and villi (G and K).