Table 1.
Electrophysiological effects of myofibroblast coupling on myocyte resting membrane potential (RMP) and action potential duration (APD90).
| Combination | Cf (pF) | Ef (mV) | Gj (nS) | ΔRMP (mV, n) | ΔAPD90 (ms, n) |
|---|---|---|---|---|---|
| 1 (fibroblast) | 6.3 | −50 | 0.3 | +0.2 ± 0.1 (40) | −5 ± 5 (25) |
| 2 (fibroblast) | 6.3 | −50 | 3.0 | +0.3 ± 0.2 (35) | −21 ± 15 (29) |
| 3 (fibroblast) | 6.3 | −25 | 0.3 | +0.9 ± 0.4 (25) | −10 ± 9 (20) |
| 4 (fibroblast) | 6.3 | −25 | 3.0 | +2.8 ± 1.6 (50) | −6 ± 6 (24) |
| 5 (myofibroblast) | 50.0 | −50 | 0.3 | +0.5 ± 0.3 (33) | −7 ± 6 (27) |
| 6 (myofibroblast) | 50.0 | −50 | 3.0 | +1.3 ± 0.7 (23) | −45 ± 15 (31) |
| 7 (myofibroblast) | 50.0 | −25 | 0.3 | +1.0 ± 0.2 (23) | −2 ± 2 (13) |
| 8 (myofibroblast) | 50.0 | −25 | 3.0 | +2.4 ± 1.6 (41) | −12 ± 13 (35) |
Coupling of rabbit ventricular myocytes to a virtual fibroblast or myofibroblast by dynamic clamp under control conditions using eight different coupling combinations slightly depolarized the myocyte resting membrane (less negative RMP) and shortened APD90 during PCL 1 s. Results are reported as mean ± SD.