Table II.
Oligonucleotide primers used for PCR
| Genes | Primers | Primer sequence (5’-3’) | Location [bp] | Size [bp] | Annealing temperature [°C] |
|---|---|---|---|---|---|
| mecR1 | Forward | acacgacttcttcggttag | 218-236 | 336 | 58 |
| Reverse | gtacaatttgggatttcact | 534-553 | |||
| mecA | Forward | gcaatcgctaaagaactaag | 553-572 | 225 | 58 |
| Reverse | aatgggaccaacataaccta | 758-777 | |||
| blaR1 | Forward | acaatgaagtagaagccgatagat | 719-742 | 489 | 55 |
| Reverse | gtcggtcaagtccaaaca | 1207-1190 | |||
| blaZ | Forward | agagatttgcctatgcttca | 311-330 | 461 | 56 |
| Reverse | agtatctccgcttttattattt | 771-750 | |||
| 16SrRNA | Forward | gttattagggaagaacatatgtg | 446-468 | 750 | 55 |
| Reverse | ccaccttcctccggtttgtcacc | 1195-1173 | |||