Table II.

Primer sequence, product size and annealing temperature used for MSP

Gene	Forward primer (F) (5’ → 3’)	Reverse primer (R) (5’ → 3’)	Annealing temperature [°C]	Product size [bp]	Ref.
p16	N1F GAAGAAAGAGGAGGGGTTGG	NR CTACAAACCCTCTAC	60	280	Palmisano et al., 2000
	M2F TTATTAGAGGGTGGGGCGGATCGC	MR GACCCCGAACCGCGACCGTAA	65	150	Herman et al., 1996
	U3F TTATTAGAGGGTGGGGTGGATTGT	UR CAACCCCAAACCACAACCATAA	60	151
DAPK	MF GGATAGTCGGATCGAGTTAACGTC	MR CCCTCCCAAACGCCGA	56	98	Esteller et al., 1999
	UF GGAGGATAGTTGGATTGAGTTAATGTT	UR CAAATCCCTCCCAAACACCAA	61	106

N – nested PCR primer, M – methylated-specific primer, U – unmethylated-specific primer