Table II.
Gene | Forward primer (F) (5’ → 3’) | Reverse primer (R) (5’ → 3’) | Annealing temperature [°C] | Product size [bp] | Ref. |
---|---|---|---|---|---|
p16 | N1F GAAGAAAGAGGAGGGGTTGG | NR CTACAAACCCTCTAC | 60 | 280 | Palmisano et al., 2000 |
M2F TTATTAGAGGGTGGGGCGGATCGC | MR GACCCCGAACCGCGACCGTAA | 65 | 150 | Herman et al., 1996 | |
U3F TTATTAGAGGGTGGGGTGGATTGT | UR CAACCCCAAACCACAACCATAA | 60 | 151 | ||
DAPK | MF GGATAGTCGGATCGAGTTAACGTC | MR CCCTCCCAAACGCCGA | 56 | 98 | Esteller et al., 1999 |
UF GGAGGATAGTTGGATTGAGTTAATGTT | UR CAAATCCCTCCCAAACACCAA | 61 | 106 |
N – nested PCR primer, M – methylated-specific primer, U – unmethylated-specific primer