Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 12;25(2):59–66. doi: 10.1093/protein/gzr058

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 3. — Functional characterization of recombinant scFvs. Complex formation between scFvs 8B9 (A), 6A5 (B) and A8 (C) and their respective cognate antigen was analyzed by SEC analysis using a Superdex 200 5/150 column (CV 3 ml). The scFv, the respective antigen and a mixture of the two were loaded to the column (in three different runs) (scFv ∼26 kD, HCV E2 ∼50 kD, BVDV E2 monomer ∼50 kD, BVDV E2 dimer ∼100 kD, complexes with antigen monomer ∼80 kD, complex with antigen dimer ∼160 kD). The peak corresponding to the free scFv in the chromatogram of the scFv-antigen complex for scFv 8B9 (A) is likely due to a molar excess of scFv used in this experiment.