FIGURE 6.

Semiquantitative immunoblot analysis of PSII and PSI subunits in wild type and gun4 mutant. Wild-type and gun4 mutant membrane fractions were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane. Different amounts of Chl corresponding to designated amounts of cells were loaded per each lane. Immunodetection was performed using polyclonal antibodies raised against the PSII subunits CP47, CP43, and D1, and the PSI subunit PsaD. ATPase band stained on the membrane with Ponceau red indicates protein loading.