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. 2008 Sep 19;283(38):26098–26106. doi: 10.1074/jbc.M803475200

FIGURE 3.

FIGURE 3.

SDS-PAGE analysis of Hep escort activity. A, molecular weight markers (lane 1) and Rosetta 2 E. coli harboring a plasmid for expressing Hep alone (pHis-Hep, lanes 2–4) and with vectors for expressing mtHsp70 (pHsp70, lanes 5–7), the 70-A chimera (p70-A, lanes 8–10), and 70ATPase (pATPase, lanes 11–13). B, molecular weight markers (lane 1) and Rosetta 2 E. coli harboring plasmids for expressing yeast Hep1 (pHep1, lanes 2–4), Hep1 and mtHsp70 (pHep1 and pHsp70, lanes 5–7), Isu2 (pHis-Isu2, lanes 8–10), and Isu2 and mtHsp70 (pHis-Isu2 and pHsp70, lanes 11–13). For each sample, 15 μg of total protein from whole cells (T) is shown as well as the soluble lysates (S) and pellets (P) derived from a sample that contained the same amount of protein before fractionation.