Nucleotide effects on mtHsp70 oligomerization. A, SDS-PAGE
analysis of purified mtHsp70: molecular weight markers (14.4, 21.5, 31, 45,
66.2, and 97.4 kDa) and 1, 3, and 10 μg of purified mtHsp70. B,
elution profiles at 4 °C for 20 μm mtHsp70 (2 ml)
chromatographed on a Superdex 200 column using HKMD buffer containing 50
μm ATP (top), 50 μm ADP
(middle), and no nucleotide (bottom).