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Hep binds to the mtHsp70 ATPase domain. A, SDS-PAGE analysis of purified mtHsp70 ATPase domain: molecular weight markers (14.4, 21.5, 31, 45, 66.2, and 97.4 kDa) and 1, 3, and 10 μg of purified 70ATPase. B, elution profiles at 4 °C for 70ATPase and Hep chromatographed alone and together on a Superdex 75 column using HKMD buffer lacking nucleotides and containing ATP or ADP (50 μm). Samples (2 ml) introduced onto the column contained 20 μm Hep and 70ATPase.
