TSA or CSE down-regulates CD9 and CD81 while up-regulating MMPs in
RAW264.7 macrophages. A, RAW264.7 cells were cultured in the
absence or presence of TSA, theophylline, and dexamethasone for 48 h.
Expressions of CD9, CD81, and integrin β1 were examined by immunoblotting
using whole cell lysates. Anti-actin blots confirm equal amounts of protein
loaded in each lane. B, RAW264.7 was cultured in the absence
(filled histograms) or presence (open histograms) of TSA.
Surface expressions of CD9, CD81, and integrin β1 were analyzed by flow
cytometry. C, A549 alveolar epithelial cells were cultured in the
absence or presence of TSA. CD9 and CD81 were immunoblotted. D,
RAW264.7 was cultured in the absence or presence of 0.1% CSE for 48 h. CD9,
CD81, and integrin β1 were immunoblotted. E, RAW264.7 was
cultured in the absence or presence of TSA. Expressions of MMP-2, MMP-9, and
MMP-12 were analyzed by RT-PCR. β-Actin amplification was internal
control.