FIGURE 4.

Thermostability of cutinase. The enzyme was incubated in 20 mm Tris-HCl (pH 8.0) at 60 °C (A) or 40 °C (B). ▵, ⋄, and □ represent the relative activity of Tfu_0882, Tfu_0883, and F. solani pisi cutinase, respectively. At various intervals, aliquots of the samples were removed and assayed for residual activity at their optimal temperature and pH. Error bars correspond to the standard deviation of three determinations.