TABLE 3.

Monomeric products released from cutin hydrolysis by recombinant cutinases and a bacterial lipase

Cutin hydrolysis by F. solani pisi cutinase, PsL, and T. fusca cutinases were incubated in 25 mm potassium phosphate buffer (pH 8.0) at temperatures of 40, 40, and 60 °C, respectively, for 18 h. The results are the averages of triplicate assays.

Cutin hydrolysis products	F. solani cutinase Area (%)	T. fusca cutinase (Tfu_0882) Area (%)	T. fusca cutinase (Tfu_0883) Area (%)	P. sp. MIS38 lipase Area (%)
Hexadecanoic acid	15.94 ± 2.62	9.27 ± 2.07	10.96 ± 2.89	24.89 ± 3.71
9-Hexadecenoic acid	0.13 ± 0.02	0.06 ± 0.02	0.09 ± 0.03	0.77 ± 0.12
Octadecanoic acid	14.99 ± 1.71	9.24 ± 3.01	8.77 ± 2.69	21.61 ± 4.09
9-Octadecenoic acid	1.74 ± 0.61	0.98 ± 0.36	1.16 ± 0.79	1.39 ± 0.67
9,12-Octadecadienoic acid	1.08 ± 0.23	1.32 ± 0.37	1.06 ± 0.23	1.33 ± 0.54
16-Hydroxyhexadecanoic acid	1.65 ± 0.31	5.66 ± 1.14	4.74 ± 0.54	NDa
10,16-Dihydroxyhexadecanoic acid	10.18 ± 1.12	8.29 ± 2.35	9.15 ± 0.97	ND
18-Hydroxyoctadeca-9-enoic acid	1.90 ± 0.34	1.03 ± 0.32	1.52 ± 0.12	ND
18-Hydroxyoctadeca-9,12-dienoic acid	7.98 ± 0.98	9.19 ± 2.25	10.91 ± 1.07	ND
9,10,18-Trihydroxyoctadecanoic acid	1.95 ± 0.42	0.99 ± 0.17	1.78 ± 0.35	ND

^aND, not detectable.