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Bcl-xL and Bcl-2 have a similar effect on the affinity of UVRAG for Beclin1. ITC titrations for UVRAG CCD in the syringe and Beclin1 BH3-CCD in the cell in the presence of a 2-fold excess of vBcl-2 (A) or Bcl-xL (B). The data were fitted to a single-site model and in the presence of Bcl-xL. n was held constant at 1.
