FIGURE 6.
Beclin1 co-localization with Bcl-xL in mitochondria is dependent on its BH3 domain. A, HeLa cells were transfected with YPet-Beclin1 (a-c), YPet-Beclin1 and mCherry-Bcl-xL (d-f), or YPet-Bcl-xL (h-i) and fixed after 6 h. Mitochondrial co-localization was assessed using MitoTracker (b and g). Panels c, f, and i are merged images of a and b, d and e, and g and h, respectively. The insets show an enlarged area (×3.4). B, representative images of COS-7 cells expressing YPet-Beclin1-(95-266) and mCherry-Bcl-xL (a-c) or YPet-Beclin1-(133-266) and mCherry-Bcl-xL (d-f). Panels c and f are merged images of a and b and or d and e, respectively.