FIGURE 7.

Beclin1 dimerizes via its CCD domain and this interaction is inhibited by UVRAG in vivo. A, COS7 total cell lysate (TCL, 400 μg) co-expressing FLAG-SBP-Beclin1 and YPet-fusion proteins as indicated were collected on 25 μl of streptavidin-agarose beads, and after washing, 10% of material was assessed by Western blotting for bound YPet proteins. Total cell lysate (5%) was used for comparison. GFP, green fluorescent protein. B, COS7 cells expressing hemagglutinin (HA)-Beclin1 BH3-CCD, YPet-Beclin1 BH3-CCD, and FLAG constructs as indicated were collected on 25 μl of streptavidin-agarose beads and processed as for A.