TABLE 1.

Summary of sedimentation equilibrium data

Protein	Partial specific volume	Mr	Fitted Mr	Concentration range	Rotor speeds	Stoichiometry	Ka
				μM	krpm		M−1
Beclin1 CCD	0.7211	16,660	32,430	3-165	20, 23, 26	Dimer
Beclin1 BH3-CCD	0.7213	20,720	41,810	2-145	16, 18, 20	Dimer
UVRAG	0.7507	10,490	11,510	2-20	30, 32, 34	Monomer
vBcl-2	0.7431	16,620	17,370	1-22	20, 23, 26	Monomer
Beclin1 BH3-CCD + vBcl-2	0.7213, 0.7431	41,440, 16,200		0.3-15	14, 16, 18	2:2	5.55 × 107
							1.13 × 106
Beclin1 BH3-CCD + Bcl-xL	0.7199	41,440, 24,290	65,070	0.5-17	14, 17, 20	2:2
Beclin1 CCD + UVRAG CCD	0.7326	10,490, 16,660	30,010	13-74	18, 21, 24	1:1