TABLE 3.

Summary of isothermal titration calorimetry data

Cell ligand	Injectant	n	Ka	ΔH	TΔS (293K)
			M−1	Kcal·mol−1	Kcal·mol−1
Beclin1 BH3-CCD	vBcl-2	0.545 ± 0.004	4.02 × 107 ± 6.8 × 106	−1.242 × 104 ± 68	−7.56
		0.929 ± 0.02	2.08 × 105 ± 3.5 × 104	−5.36 × 103 ± 276	6.04
Beclin1 BH3 peptide	vBcl-2	0.964 ± 0.02	1.77 × 105 ± 1.0 × 104	−7.10 × 103 ± 161	−0.20
Beclin1 BH3-CCD	Bcl-xL	0.978 ± 0.004	9.08 × 105 ± 4.6 × 104	−1.09 × 104 ± 63	−9.82
Beclin1 BH3 peptide	Bcl-xL	0.952 ± 0.002	1.54 × 106 ± 3.0 × 104	−1.32 × 104 ± 29	−16.6
Beclin1 BH3-CCD	UVRAG CCD	1.02 ± 0.01	2.04 × 105 ± 1.2 × 104	1.12 × 104 ± 240	62.4
Beclin1 BH3-CCD (2× UVRAG CCD)	vBcl-2	0.976 ± 0.017	4.59 × 105 ± 5.6 × 104	−500 ± 118	8.83
Beclin1 BH3-CCD (2× UVRAG CCD)	Bcl-xL	0.954 ± 0.002	1.16 × 106 ± 2.2 × 104	−1.14 × 104 ± 32	−11.0
Beclin1 BH3-CCD (2× vBcl-2)	UVRAG CCD	1.06 ± 0.06	4.99 × 104 ± 8.4 × 103	1.58 × 104 ± 1680	75.5
Beclin1 BH3-CCD (0.5× vBcl-2)	UVRAG CCD	1.02 ± 0.02	1.85 × 105 ± 1.2 × 104	1.24 × 104 ± 312	66.3
Beclin1 BH3-CCD (2× Bcl-xL)	UVRAG CCD	1.0	4.66 × 104 ± 1.8 × 103	5.53 × 104 ± 1254	210