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Carbamylation inhibition assay using KOCN. At the indicated time points, aliquots of wild-type FVIIa (♦), FVIIaD212N (▴), and FVIIaDVQ (▪) were withdrawn and the residual activity measured. The curves show the result of a representative experiment. Similar results were obtained in two additional experiments in which samples were withdrawn at time points different from the experiment presented (data not shown).
