FIGURE 2.

rTbHK1 and rTbHK2 formoligomers. A, native gel(4% acrylamide) analysis of rTbHK1 (50 ng; lane1) after treatment (30 min, room temperature) with DMSO (lane 2), myristate (500 μm; lanes 3–5), or LND (10 mm; lanes 8–10) in the presence of 5 mm ATP/Mg²⁺ (lanes 3 and 8), 5 mm glucose (lanes 4 and 9), or no substrate (lanes 5 and 10) is shown. Additionally, untreated rTbHK1 (lane 6), untreated rTbHK2 (lane 11), DMSO treated rTbHK1 (lane 7), and rTbHK2 treated with ATP and myristate (lane 12) are included. Samples were then resolved and silver-stained. B, treatment of rTbHK1 oligomers with increasing salt can disrupt the complex. rTbHK1 (50 ng) was incubated with increasing NaCl(upper panels), KCl(middle panels), or MgCl₂ (lower panels), and the impact was assessed by native gel (upper gel) or 10% SDS-polyacrylamide gel (lower gel). Salt concentrations were 250 mm, 500 mm, 1 m, and 2.5 m. Aberrant migration (upon SDS-PAGE) was likely due to the high salt concentrations.