FIGURE 5.

Reassembly of mixed oligomers reveals that TbHK2 has HK activity. A, schematic representation for assembly of mixed complexes. B, hexokinase activity of untreated, DMSO-treated, or myristate-dissociated and reassembled rTbHK1 and rTbHK2 (left panel) and rTbHK1(S160A) (right panel). Equal amounts (250 ng) of rTbHK1 and rTbHK2 were used throughout. Please note that the differences in HK activities of DMSO-treated rTbHK1 in the left and right panels were due to differences in the specific activity of proteins used.