FIGURE 3.

Expression and purification of the His-tagged BmSUC1 protein. Recombinant BmSUC1 protein with the His tag at the C terminus was expressed using a baculovirus expression system. BmSUC1 was purified from the medium of virus-infected cells by nickel chromatography. Protein samples were analyzed by SDS-PAGE and stained with CBB (a), and the same samples were analyzed by immunoblot with the anti-His antibody (b). The molecular masses of the protein standards are shown on the left, and the estimated molecular mass of BmSUC1 is shown on the right. The following are the protein samples used. Mock, the medium from mock-infected cells; WT, the medium from parental AcMNPV-infected cells; BmSUC1-His, the medium from cells infected with the recombinant AcMNPV that expressed BmSUC1 protein; purified, the purified BmSUC1 protein.